NMR studies of [U‐13C]cyclosporin A bound to human cyclophilin B

FEBS Letters

Neri, Placido¹  Zydowsky, Lynne D.²  Fesik, Stephen W.¹  Gemmecker, Gerd¹  Walsh, Christopher T.² 

[1] Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, IL 60064, USA;Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA

关键词: Cyclophilin B;    Cyclosporin A;    NMR;    Conformation;    CyP;    cyclophilin (A or B);    CsA;    cyclosporin A;    NOE;    nuclear Overhauser effect;    HSQC;    heteronuclear single quantum correlation;    HMQC-NOESY;    heteronuclear multiple quantum correlation-nuclear Overhauser effect spectroscopy;   

DOI  :  10.1016/0014-5793(91)81258-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

NMR data (¹H and ¹³C chemical shifts, NOEs) on [[U-¹³C]cyclosporin A bound to cyclophilin B were compared to previously published data on the [U-¹³C]CsA/CyPA complex [Fesik et al., (1991) Biochemistry 30, 6574–6583]. Despite only 64% sequence identity between CyPA and CyPB, the conformation and active site environment of CsA when bound to CyPA and CyPB are nearly identical as judged by the similarity of the NMR data.

【 授权许可】

Unknown