A → Z transition in the synthetic hexanucleotide (dCdGfl)3

FEBS Letters

Fazakerley, G.V.¹  Guschlbauer, W.¹  Uesugi, S.²  Ikehara, M.²  Izumi, A.² 

[1] Service de Bochimie, Centre d'Etudes Nucléaires de Saclay, F-91191 Gif-sur- Yvette Cedex, France;School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan

关键词: NMR;    NOE;    2́-Deoxy-2́-fluoronucleoside;    CD;    Conformation;    dGfl;    2́-deoxy-2́-fluoroguanosine;    NOE;    nuclear Overhauser effect;    CD;    circular dichroism;   

DOI  :  10.1016/0014-5793(85)80334-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

500 MHz proton NMR and NOE measurements on (dCdGfl)₃ show that at very low ionic strength the hexanucleotide adopts an A-DNA conformation, whereas at high salt concentrations a Z-form is found. At intermediate salt concentrations the two species are in slow exchange on the proton NMR time scale. This transition was also observed by characteristic changes in the CD spectra.

【 授权许可】

Unknown