Pulmonary surfactant‐associated polypeptide SP‐C in lipid micelles: CD studies of intact SP‐C and NMR secondary structure determination of depalmitoyl‐SP‐C(1–17)

FEBS Letters

Johansson, Jan¹  Wüthrich, Kurt¹  Szyperski, Thomas¹ 

[1] Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule-Hönggerberg, CH-8093 Zürich, Switzerland

关键词: NMR;    Polypeptide conformation;    Lipid-associated polypeptide;    Pulmonary surfactant;    Surfactant polypeptide-C;    SP-C;    surfactant-associated polypeptide C;    depalmitoyl-SPC(1–17);    N-terminal heptadecapeptide fragment of SP-C;    DPC;    dodecylphosphocholine;    DPPC;    1;    2-dipalmitoyl-sn-glycero-3-phosphocholine;    TSP;    [2;    2;    3;    3-2H4]-trimethylsilyl-propionate;    RDS;    respiratory distress syndrome;    CD;    circular dichroism;    NMR;    nuclear magnetic resonance;    ppm;    parts per million;    2QF-COSY;    two-dimensional two-quantum filtered correlation spectroscopy;    TOCSY;    two-dimensional total correlation spectroscopy;    NOE;    nuclear Overhauser enhancement;    NOESY;    two-dimensional nuclear Overhauser enhancement spectroscopy;   

DOI  :  10.1016/0014-5793(95)00216-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The surfactant-associated polypeptide C (SP-C) is a 35-residue lipopolypeptide which is essential for the function of surfactants used for therapy of infant respiratory distress. Modeling based on the recently determined nuclear magnetic resonance (NMR) structure of native SP-C in an organic solvent showed that SP-C could readily insert into fluid 1,2-dipalmitoyl-sn-glycero-3-phosphocholine bilayers. The present paper describes further physical-chemical studies of intact SP-C and its N-terminal 17-residue polypeptide fragment, depalmitoyl-SPC(1–17), in the presence of dodecylphosphocholine micelles. The results obtained provide a link between the NMR solution structure and the behaviour of SP-C in an ordered lipid environment, and thus present new insights for rational design of SP-C analogs for therapeutic purposes.

【 授权许可】

Unknown