A cyclic‐AMP‐gated conductance in cochlear hair cells

FEBS Letters

Zhainazarov, Aslbek B.¹  Tavartkiladze, George A.³  Kalamkarov, Grigorii R.¹  Kolesnikov, Stanislav S.¹  Rebrik, Tatyana I.² 

[1] Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region 142292, USSR;Institute of Chemical Physics, USSR Academy of Sciences, Kosygin str., 4, Moscow 117334, USSR;All-Union Scientific Center for Audiology and Health, 1905 g. str,, 10a 123022, Moscow, USSR

关键词: Cochlear hair cell;    cAMP-activated channel;    Patch clamp technique;   

DOI  :  10.1016/0014-5793(91)81251-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The patch clamp technique was used to record cAMP-dependent currents of the guinea pig cochlear hair cell plasma membrane. Data obtained indicate that the channels passing this current are moderately selective for monovalent cations and are effectively blocked by L-cis-diltiazem and reversibly blocked by 1 mM Mg²⁺ or Ca²⁺. The single-channel unit conductance estimated in the absence of divalent cations is about 16 pS. The results demonstrate that cyclic nucleotide-dependent channels of cochlear hair cells are virtually identical to the photoreceptor and olfactory ones.

【 授权许可】

Unknown