‘Neuron‐specific’ protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth state

FEBS Letters

Pula, Grazia²  Sorci, Guglielmo²  Ceccarelli, Paolo²  Giambanco, Ileana²  Bocchini, Virginia¹  Bianchi, Roberta²  Antonioli, Sabrina²  Donato, Rosario² 

[1] Section of Cellular and Molecular Biology, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Cas. Post. 81, 06100 Perugia Succ. 3, Italy;Section of Anatomy Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Cas. Post. 81, 06100 Perugia Succ. 3, Italy

关键词: Protein gene product 9.5 (PGP 9.5);    Glial cell;    Immunoblotting;    Immunocytochemistry;   

DOI  :  10.1016/0014-5793(91)81242-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and second post-plating day. Only a faint PGP 9.5 immunoreactivity can be observed in glioma cells after the eleventh post-plating day, i.e. about one week after confluency has been reached. The present results suggest that PGP 9.5 in cultured glial cells is maximally expressed during the growth phase and that the protein could play a role during brain development in glial cells, in reactive gliosis, or in tumorigenesis of the glial lineage.

【 授权许可】

Unknown