Binding sites in fibronectin for an enterotoxigenic strain of E. coli B342289c

FEBS Letters

Speciale, Livianna¹  Petersen, Torben E.²  Bozzini, Silvia¹  Visai, Livia¹  Speziale, Pietro¹ 

[1] Department of Biochemistry, University of Pavia, Via Bassi, 21, 27100 Pavia, Italy;Department of Molecular Biology and Plant Physiology, University of Aarhus, C.F. Mollers Alle' 130, DK-8000 Arhus C., Denmark

关键词: Fibronectin;    Fibronectin fragment;    Bacterium;    Receptor;   

DOI  :  10.1016/0014-5793(91)81238-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The binding of fibronectin and fibronectin fragments to the enterotoxigenic strain E. coli B34289c was studied. E. coli cells bound to two distinct sites of fibronectin, one being the N-terminal domain, which also contains the binding sites for staphylococci and streptococci, and the other located within the central heparin binding region. In addition, the N-terininal and the heparin binding domain mediated the attachment of bacteria in a solid phase binding assay. E. coli cells expressed two classes of receptors, the first, a 17 kDa protein, recognized by the N-terminal fragment and the second, having a mol. mass of 55 kDa, which interacts with the internal heparin binding domain. Bacterial receptors, which bind the N-terminal end of fibronectin, may be structurally related.

【 授权许可】

Unknown