Studies of extracellular fibronectin matrix formation with fluoresceinated fibronectin and fibronectin fragments

FEBS Letters

Koteliansky, V.E.¹  Metsis, M.L.¹  Chernousov, M.A.¹ 

[1] Laboratory of Molecular and Cellular Cardiology, Institute of Experimental Cardiology, Cardiology Research Center of the USSR, Academy of Medical Sciences, 3rd Cherepkovskaya str. 15 A, Moscow 121552, USSR

关键词: Fibronectin;    Fibronectin fragment;    Extracellular matrix formation;   

DOI  :  10.1016/0014-5793(85)80811-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fluorescein isothiocyanate conjugated human plasma fibronectin, 70-kDa collagen-binding, 60-kDa central, 60-kDa heparin-binding, 180-kDa heparin, collagen-binding fibronectin fragments and gelatin were used to study extracellular fibronectin matrix formation. Exogenous fibronectin, gelatin, 70-kDa collagen-binding and 180-kDa heparin, collagen-binding fragments were shown to be able to bind specifically to preexisting extracellular matrix of living fibroblasts. The results suggest that: (i) Fibronectin matrix formation may occur through a self-assembly process; (ii) the NH₂-terminal part of fibronectin is responsible for fibronectin-fibronectin interaction during fibronectin fibril formation; (iii) plasma fibronectin may be the source for tissue fibronectin.

【 授权许可】

Unknown