Strong expression of foreign genes following direct injection into fish muscle

FEBS Letters

Goldspink, Geoffrey¹  Umeda, Patrick K.³  Butterworth, Peter²  Chang, Kin-Chow¹  Hansen, Ekkehard¹  Fernandes, Kenneth¹ 

[1] Unit of Veterinary Molecular and Cellular Biology, The Royal Veterinary College, University of London, Royal College Street London NW1 OTU, UK;University of Surrey, Guildford, Surrey, UK;Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, USA

关键词: Gene injection;    Fish muscle;    Gene expression;    MxA;    Cyprinus carpio;   

DOI  :  10.1016/0014-5793(91)81229-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report here for the first time direct injection of genes into fish muscle in vivo. Plasmids used contain either SV40 early promoter, rabbit β-cardiac myosin heavy chain promoter, human MxA promoter of an artificial promoter, fused to a chloramphenicol acetyltransferase (CAT) or β-galactosidase reporter gene. CAT assays revealed that most gene constructs were highly expressed. Histochemical analysis showed that β-galactosidase was strongly expressed at the site of injection within muscle fibres. This method provides an excellent system for testing expression of gene constructs, including those of mammalian origin, in fish muscle in vivo and has the potential for fish vaccination.

【 授权许可】

Unknown