FEBS Letters | |
Presence of phospholipase C in coated vesicles from bovine brain Dual regulatory effects of GTP‐analogs | |
Martín, Mairena1  González-Calero, Gloria1  Cubero, Ana1  Ros, Manuel1  | |
[1] Departamento de Inorgánica, Orgánica y Bioquímica, Facultad de Químicas, Universidad de Castilla-La Mancha, Ciudad Real 13071, Spain | |
关键词: Coated vesicle; Phospholipase C; G-protein; Guanine nucleotide; Gpp(NH)p; guanyl-5-yl-imidodiphosphate; GTPγS; guanosine 5′-(γ-thio)triphosphate; Ptd Ins P2; phosphatidylinositol 4; 5-bisphosphate; PLC; phospholipase C; | |
DOI : 10.1016/0014-5793(91)81216-U | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Bovine brain coated vesicles display free calcium-dependent phospholipase C activity. Gpp(NH)p and GTPγS inhibited phospholipase C at nanomolar concentrations. Increasing concentrations of Gpp(NH)p and GTPγS reversed the inhibitory effects and stimulated phospholipase C activity. Preincubation of coated vesicles with pertussis toxin blocked the poorly-hydrolyzable GTP-analogs' inhibitory effects on phospholipase C. These data indicate that guanine nucleotides exert a dual regulatory control of phospholipase C in coated vesicles and that the inhibitory pathway is mediated by a pertussis toxin-sensitive G-protein.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020295342ZK.pdf | 625KB | download |