Presence of phospholipase C in coated vesicles from bovine brain Dual regulatory effects of GTP‐analogs

FEBS Letters

Martín, Mairena¹  González-Calero, Gloria¹  Cubero, Ana¹  Ros, Manuel¹ 

[1] Departamento de Inorgánica, Orgánica y Bioquímica, Facultad de Químicas, Universidad de Castilla-La Mancha, Ciudad Real 13071, Spain

关键词: Coated vesicle;    Phospholipase C;    G-protein;    Guanine nucleotide;    Gpp(NH)p;    guanyl-5-yl-imidodiphosphate;    GTPγS;    guanosine 5′-(γ-thio)triphosphate;    Ptd Ins P2;    phosphatidylinositol 4;    5-bisphosphate;    PLC;    phospholipase C;   

DOI  :  10.1016/0014-5793(91)81216-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bovine brain coated vesicles display free calcium-dependent phospholipase C activity. Gpp(NH)p and GTPγS inhibited phospholipase C at nanomolar concentrations. Increasing concentrations of Gpp(NH)p and GTPγS reversed the inhibitory effects and stimulated phospholipase C activity. Preincubation of coated vesicles with pertussis toxin blocked the poorly-hydrolyzable GTP-analogs' inhibitory effects on phospholipase C. These data indicate that guanine nucleotides exert a dual regulatory control of phospholipase C in coated vesicles and that the inhibitory pathway is mediated by a pertussis toxin-sensitive G-protein.

【 授权许可】

Unknown