cDNA sequence of a Drosophila melanogaster gene, Dfur1, encoding a protein structurally related to the subtilisin‐like proprotein processing enzyme furin

FEBS Letters

Zhang, Young¹  van de Ven, Wim J.M.¹  Roebroek, Anton J.M.¹  Pauli, Ilse G.L.¹ 

[1] Molecular Oncology Section, Center for Human Genetics, University of Leuven, Herestraat 49, B-3000 Leuven, Belgium

关键词: Subtilisin-like proprotein processing enzyme;    Furin;    Dfurin 1;    Drosophila melanogaster;   

DOI  :  10.1016/0014-5793(91)81052-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Screening a genomic library of Drosophila melanogaster DNA with a human fur cDNA probe resulted in the isolation of DNA clones that apparently belonged to two different DNA regions of the Drosophila genome. Subsequently, corresponding Drosophila cDNA clones were isolated. Nucleotide sequence analysis indicated that these cDNA clones originated from two different genes, which were called Dfur1 and Dfur2. From overlapping Dfur1 cDNA clones, a composite cDNA could be constructed and analysis of its nucleotide sequence revealed the coding sequence for a protein of 899 amino acid residues. This protein, designated Dfurin1, exhibited striking sequence homology to human furin and contained the same protein domains except for the cysteine-rich region. Furthermore, unlike human furin. Dfurin1 possessed an extended amino-terminal region in which a potential transmembrane anchor was present.

【 授权许可】

Unknown