Purification and characterization of N‐acyl‐D‐glutamate deacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A‐6

FEBS Letters

Moriguchi, Mitsuaki¹  Sakai, Kenji¹  Kido, Hiroyuki¹  Sonoda, Yuji¹  Imamura, Kazuyuki¹ 

[1] Department of Environmental Chemistry and Engineering, Oita University, Oita 870-11, Japan

关键词: N-Acyl-D-glutamate deacylase;    Alcaligenes;    D-Glutamate;   

DOI  :  10.1016/0014-5793(91)80904-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The purification and properties of N-acyl-D-glutamate deacylase from the cell extracts of Alcaligenes xylosoxydans subsp. xylosoxydans A-6 were studied. The two active fractions (peaks I and II) were obtained by a Mono Q column chromatography. The predominant enzyme (peak I) has been purified, 1960-fold to homogeneity and characterized. The enzyme was a monomer with a molecular weight of 59 000. The optimum pH and the isoelectric point were 8.0 and 5.5, respectively. The enzyme catalyzed the hydrolysis of N-acyl derivatives of D-glutamate. The K _ms for N-acetyl, N-butyryl and N-propionyl derivatives of D-glutamate were 0.129, 0.066 and 0.01 mM, respectively.

【 授权许可】

Unknown