Purification and characterization of β-1,3-xylanase from a marine bacterium, Alcaligenes sp. XY-234

The Journal of General and Applied Microbiology

Toshiyoshi Araki¹  Tatsuo Morishita¹  Naotake Inoue¹ 

[1] Faculty of Bioresources, Mie University

关键词: Alcaligenes;    marine bacterium;    β-1;    3-xylan;    β-1;    3-xylanase;   

DOI  :  10.2323/jgam.44.269

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

A β-1,3-xylanase-producing bacterium, Alcaligenes sp. XY-234, was isolated from the marine environment. The organism produced endo-1,3-β-xylanase at a high level in the culture fluid. The enzyme was purified 292-fold by ammonium sulfate precipitation and several column chromatographies. The final enzyme preparation appeared to be homogeneous on disc gel electrophoresis and SDS-PAGE with a molecular mass of 59 kDa, and the pl was 4.0. The enzyme hydrolyzed β-1,3-xylan and larger xylooligosaccharides than xylobiose to give several xylooligosaccharides, but it could not hydrolyze xylobiose, p-nitrophenyl-β-D-xyloside, and β-1,4-xylan. The Km of the enzyme was 4.0 mg/ml. Optimal pH and temperature were 7.5 and 40°C, respectively. It was stable from pH 6.0 to 10 and at a temperature of less than 40°C. The enzyme was strongly inhibited by 1 mM HgCl2, AlCl3, CuCl2, FeCl3, HgCl2, Pb(CH3COO)2, and N-bromosuccinimide.

【 授权许可】

Unknown