Maltose adaptive mutant of heterotrophic marine bacterium, Alteromonas espejiana Bal-31: Changes in the growth and the induction of extracellular α-amylase

The Journal of General and Applied Microbiology

Wu-Fu Tong²  Shing-Lon Ho¹  Tyy-Huoy Lee²  Hsueh-Mei Chou² 

[1] Department of Agronomy, National Chia-Yi University;Department of Life Science, National Taiwan Normal University

关键词: Alteromonas espejiana Bal-31;    carbon nutrient;    extracellular α-amylase;    marine bacterium;    mutation;   

DOI  :  10.2323/jgam.51.165

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Growth of the heterotrophic marine bacterium, Alteromonas espejiana Bal-31 was inhibited in the presence of sucrose, maltose and even glucose, but not with starch. Extracellular α-amylase was induced with a lag phase of 2 h in the presence of starch. In contrast, cell growth of the S2a mutant was not affected by the addition of maltose, and starch was ineffective in the induction of extracellular α-amylase in this mutant. Activity of extracellular α-amylase was induced from the S2a mutant with a 4-h lag phase in the presence of maltose, and the high level of enzyme activity was maintained for at least 24 h. Activity of α-amylase induced by both wild type starch and S2a mutant maltose cultures were mainly observed in extracellular locations. This activity could be stopped by tetracycline treatment, indicating that enzyme induction was dependant on gene expression and not on enzyme protein secretory mechanisms. Our results showed that the mutation in S2a changed the growth and the modulation of the specific α-amylase in response to carbon nutrients.

【 授权许可】

Unknown