Chemical detection of natural peptides by specific structures Isolation of chicken galanin by monitoring for its N‐terminal dipeptide, and determination of the amino acid sequence

FEBS Letters

Mutt, Viktor²  Carlquist, Mats³  Norberg, Åke²  Jörnvall, Hans¹  Sillard, Rannar² 

[1]Department of Chemistry I, Karolinska Institute, Box 60400, S-10401 Stockholm, Sweden

[2]Department of Biochemistry II, Karolinska Institute, Box 60400, S-10401 Stockholm, Sweden

[3]Karo Bio AB, P.O. Box 4032, S-14104 Huddinge, Sweden

关键词: Chemical assay;    Neuropeptide;    Galanin;    Peptide amide;    Chicken;   

DOI  :  10.1016/0014-5793(91)81023-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated galanin from chicken intestine by monitoring for the N-terminal glycyltryptophan, which constitutes a conserved part characteristic of the peptide. This monitoring method complements that previously used for C-terminal amide detection and proves chemical monitoring of specific structures to be useful. The isolation allowed determination of the structure, which was found to be unidentical to any of the known galanins. However, N-terminal pentadecapeptide parts are identical, showing this segment to be of special importance. In addition to common substitutions at positions 16, 18, 23, 26 and 29, chicken galanin has phenylalanine at position 28, where all known mammalian galanins have leucine.

【 授权许可】

Unknown