Melatonin binding proteins identified in the rat brain by affinity labeling

FEBS Letters

Laudon, Moshe¹  Zisapel, Nava¹ 

[1] Department of Biochemistry, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel

关键词: Melatonin;    Iodomelatonin;    Affinity labeling;    Brain;   

DOI  :  10.1016/0014-5793(91)81013-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N-Bromoacetyl-2-iodo-5-methoxytryptamine (BIM), a novel derivative of the biologically active melatonin analog, 2-iodomelatonin, was prepared and used to identify melatonin binding proteins in rat brain synaptosomes. Incubation of the synaptosomes with BIM resulted in a time and concentration dependent, irreversible inhibition of 2-[¹²⁵I]iodomelatonin binding. In parallel, the radioactive form of BIM, N-bromoacetyl-2-[¹²⁵I]iodo-5-methoxytryptamine [¹²⁵I]BIM) became incorporated into the synaptosomes. The incorporation of [¹²⁵I]BIM was inhibited by BIM, 2-iodomelatonin and melatonin but not by 5-methoxytryptamine or N-acetyl serotonin. [¹²⁵I]BIM became covalently attached to three polypeptides with apparent molecular weight values of 92, 55 and 45 kDa; the labeling of all three proteins was markedly inhibited by melatonin. These results indicate that the 92, 55 and 45 kDa polypeptides are melatonin binding proteins.

【 授权许可】

Unknown