Developmental expression of G proteins that differentially modulate adenylyl cyclase activity in mouse brain

FEBS Letters

Klee, Werner A.²  Streaty, Richard A.²  Loh, Y.Peng¹  Rius, R.Adrian¹ 

[1] Section on Cellular Neurobiology, Laboratory of Developmental Neurobiology, National Institute of Child Health and Human Development, Bethesda, MD 20892, USA;Laboratory of Molecular Biology, National Institute of Mental Health, Bethesda, MD 20892, USA

关键词: Development (Mouse brain);    G protein;    Alternative splicing;    Adenylyl cyclase;    Opiate receptor;    G proteins (Gs;    Gi1;    Gi2 and Go;    guanine nucleotide regulatory proteins;    E;    embryonic day;    EGTA;    [ethylenebis(oxyethylenetrilo)]tetracetic acid;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    Gpp[NH]p);    guanyl-5′-yl-imidodiphosphate;    TBST;    0.05% Tween in Tris-buffered siline;   

DOI  :  10.1016/0014-5793(91)81001-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Changes in the relative abundance of the G protein α subunits were observed during early mouse development G_sa was almost exclusively present as a large form (G_s-1) in prenatal brain. Postnatally with a substantial increase in Gpp[NH]p stimulated adenylyl cyclase activity, the small form (G_?? increased in amount while G_s-1 decreased. These results suggest that the G_s-1 may be the more effective cyclase activator and that changes in alternative splicing are developmentally regulated. G_i1 and G_o appeared before birth whereas Gi₂, developed postnatally. Opiate stimulation of GTPase and inhibition of adenylyl cyclase were fully expressed prenatally.

【 授权许可】

Unknown