Reversible expression of sm α‐actin protein and sm α‐actin mRNA in cloned cerebral endothelial cells

FEBS Letters

Amberger, A.³  Kocher, O.¹  Bauer, H.⁴  Tontsch, U.³  Gabbiani, G.²  Bauer, H.C.³ 

[1] Dept. Pathol. School of Medicine Yale University, New Haven, Connecticut, USA;Dept. de Pathologie, Univ. Geneve. 1211 Geneve 4, Switzerland;Institut für Molekularbiologie der Österr. Akad. der Wissenschaften. A-5020 Salzburg, Austria;Institut für Zoologie, Universität Salzburg, A-5020 Salzburg, Austria

关键词: Cerebral endothelial cell;    Phenotypic switch;    Smooth muscle α-actin;    ECGF;    Endothelial cell growth factor;    ECGS;    endothelial cell growth supplement;    FGF;    fibroblast growth factor;    TGF;    transforming growth factor;   

DOI  :  10.1016/0014-5793(91)80056-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of smooth muscle (sm) α-actin was studied in cloned capillary cerebral endothelial cells of two phenotypes. Type I cells were cultured in medium containing 10% FCS, heparin and ECGS (or α-ECGF) and stained positive for a specific endothelial cell marker (Bandeiraea simplicifolia). Depletion of heparin and ECGS resulted in a smooth muscle-like appearance after 2–3 days. Cells of this phenotype, (type II) stained positive for the endothelial cell marker and for sm α-actin. In contrast to type I cells, type II cells expressed sm α-actin protein and mRNA as evidenced by Immunoblots and Northern blots. This phenotypic switch was shown to be reversible and so was the expression of sm α-actin.

【 授权许可】

Unknown