期刊论文详细信息
FEBS Letters | |
Purification and characterization of E37, a major chloroplast envelope protein | |
Block, Maryse A.1  Joyard, Jacques1  Douce, Roland1  | |
[1] DBMS/PCV, UA no. 576 au CNRS, CENG et UJF85X, F38041 Grenoble, France | |
关键词: Chloroplast envelope; Inner envelope membrane; E37 polypeptide; Membrane protein purification; Amino acid sequence; Spinach chloroplast; CAPS; 3-(cyclohexylamino)-1-propanesulfonic acid; | |
DOI : 10.1016/0014-5793(91)80042-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have purified to homogeneity E37, the second major polypeptide of the inner membrane of the chloroplast envelope. The protein was retained on a Mono S column at pH 7, indicating it is a basic protein. After cyanogen cleavage, the protein was partially sequenced at 2 different sites. The sequence is compared with the deduced amino acid sequence of a cDNA coding for a 37 kDa envelope polypeptide recently published by Dreses-Werringloer et al. (Eur. J. Biochem. (1991) 195, 361-368.)
【 授权许可】
Unknown
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