Identification and nucleotide sequence determination of the gene responsible for Ogawa serotype specificity of V. cholerae 01

FEBS Letters

Ito, Teruyo¹  Yokota, Takeshi¹  Hiramatsu, Keiichi¹  Ohshita, Yoshihiro¹ 

[1] Department of Bacteriology, Faculty of Medicine, Juntendo University, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: O-antigen;    Serotype Ogawa;    V. cholerae;    Biosynthesis of lipopolysaccharide;    Sequence;    Deletion plasmid;   

DOI  :  10.1016/0014-5793(91)80964-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The gene encoding a protein of 27 kDa, which is specifically expressed in Vibrio cholerae of serotype Ogawa, was identified and its nucleotide sequence determined. The plasmid carrying this gene was found to convert serotype specificity from Inaba to Ogawa when introduced into the Escherichia coli DH5(pVCI112) cell which harbors a cloned 20-kilobase genomic DNA fragment of V. cholerae NIH35A3 and expresses the 01 antigen of Inaba serotype.

【 授权许可】

Unknown