FEBS Letters | |
Photoaffinity labeling of dog pancreas microsomes with 8‐azido‐ATP inhibits association of nascent preprolactin with the signal sequence receptor complex | |
Mayinger, Peter1  Klappa, Peter1  Zimmermann, Richard1  Zimmermann, Maria1  | |
[1] Institut für Physiologische Chemie und Physikalische Biochemie der Universität München, Goethestr. 33, D-8000 München 2, Germany | |
关键词: Protein transport; Endoplasmic reticulum; | |
DOI : 10.1016/0014-5793(91)80949-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Transport of bovine preprolactin into dog pancreas microsomes involves a microsomal protein which is sensitive to photoaffinity labeling with azido-ATP and which is distinct from the ATP-binding protein, immunoglobulin heavy chain binding protein. Here we addressed the question of what stage of preprolactin transport is affected. Thus a nascent presecretory protein which is related to preprolactin, termed ppl-86mer, was employed. Here we show that the nascent preprolactin did not become associated with the α-subunit of the signal sequence receptor complex after photoaffinity labeling of microsomes with azido-ATP. Therefore, we conclude that the microsomal protein which is sensitive to photoaffinity labeling with azido-ATP acts prior to the signal sequence receptor complex.
【 授权许可】
Unknown
【 预 览 】
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