Photoaffinity labeling of dog pancreas microsomes with 8‐azido‐ATP inhibits association of nascent preprolactin with the signal sequence receptor complex

FEBS Letters

Mayinger, Peter¹  Klappa, Peter¹  Zimmermann, Richard¹  Zimmermann, Maria¹ 

[1] Institut für Physiologische Chemie und Physikalische Biochemie der Universität München, Goethestr. 33, D-8000 München 2, Germany

关键词: Protein transport;    Endoplasmic reticulum;   

DOI  :  10.1016/0014-5793(91)80949-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transport of bovine preprolactin into dog pancreas microsomes involves a microsomal protein which is sensitive to photoaffinity labeling with azido-ATP and which is distinct from the ATP-binding protein, immunoglobulin heavy chain binding protein. Here we addressed the question of what stage of preprolactin transport is affected. Thus a nascent presecretory protein which is related to preprolactin, termed ppl-86mer, was employed. Here we show that the nascent preprolactin did not become associated with the α-subunit of the signal sequence receptor complex after photoaffinity labeling of microsomes with azido-ATP. Therefore, we conclude that the microsomal protein which is sensitive to photoaffinity labeling with azido-ATP acts prior to the signal sequence receptor complex.

【 授权许可】

Unknown