Differential expression of GABAA receptor α‐subunits in rat brain during development

FEBS Letters

Whiting, P.¹  Cox, P.¹  Gillard, N.P.¹  McKernan, R.M.¹ 

[1] Department of Biochemistry, Neuroscience Research Centre, Merck Sharp and Dohme Research Laboratories, Terlings Park, Eastwick Road, Harlow, Essex, CM20 2QR, UK

关键词: GABAA receptor;    α-Subunit;    Polyclonal antiserum;    Ontogeny;    Rat brain;   

DOI  :  10.1016/0014-5793(91)80936-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Unique cytoplasmic loop regions of the α₁, α₂, α₃ and α₅ subunits of the GABA_A receptor have been expressed in E. coli and used to generate polyclonal antisera specific for these subunits. The antibodies identify proteins by SDS-polyacrylamide gel electrophoresis and western blotting of molecular size 51 kDa, 53 kDa, 59 kDa and 55 kDa, respectively, which show differential patterns of expression during development. Whereas the α₂ and α₃ subunits are present at early stages, the expression of α₁ and α₅ subunits is low at birth and increases with age. This differential expression could be correlated with previous studies examining the developmental expression of BZ1 and BZ2 benzodiazepine binding sites.

【 授权许可】

Unknown