Determination of the branch location of extra N‐acetyllactosamine units in sialo N‐linked tetraantennary oligosaccharides

FEBS Letters

Vliegenthart, Johannes F.G.¹  van Dedem, Gijs W.K.²  Hokke, Cornelis H.¹  Kamerling, Johannis P.¹ 

[1] Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands;Diosynth BV, Oss, The Netherlands

关键词: Recombinant erythropoietin;    Extra N-acetyllactosamine unit;    1H-NMR spectroscopy;    Neu5Ac;    N-acetylneuraminic acid;    Fuc;    fucose;    EPO;    erythropoietin;    PNGase-F;    peptide-N 4-(N-acetyl-β-glucosaminyl)asparagine amidase F;    HPAE-PAD;    high-pH anion-exchange pulsed amperometric detection;   

DOI  :  10.1016/0014-5793(91)80931-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-β-galactosidase, followed by treatment with N-acetyl-β-glucosaminidase, yielding products which could be analysed by ¹H-NMR spectroscopy, thereby giving conclusive data about the location of the extra units in the intact structures.

【 授权许可】

Unknown