期刊论文详细信息
American Journal of Translational Research
Exploring potential biomarkers of early stage esophageal squamous cell carcinoma in pre- and post-operative serum metabolomic fingerprint spectrum using 1H-NMR method
Yanyan Liu1  Zhongxian Yang2  Limin Ma3 
[1] Department of Cardiothoracic Surgery, The Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China;Department of Medical Imaging Center, The Second Affiliated Hospital, Shantou University Medical College, Shantou 515041, Guangdong, China;Department of Public Health, Shantou University Medical College, No. 22 Xinling Road, Shantou 515041, Guangdong, China
关键词: Esophageal squamous cell carcinoma;    metabolomics;    biomarker;    1H-NMR spectroscopy;   
DOI  :  
学科分类:医学(综合)
来源: e-Century Publishing Corporation
PDF
【 摘 要 】

Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent types of upper gastrointestinal malignancy. Here, we used 1H nuclear magnetic resonance spectroscopy (1H-NMR) to identify potential pre- and post-operative serum biomarkers in patients with early stage ESCC using metabolomic fingerprint spectrum. Serum samples from preoperative patients with ESCC (ESCC, n = 25), postoperative patients with ESCC (PO, n = 24), and controls (n = 40) were analysed using 1H-NMR spectroscopy. Using orthogonal partial least squares-discriminant analysis, 31 altered serum metabolites were successfully identified among the three groups. These metabolites are indicative of the changes that occur with glycometabolism, the metabolism of fatty acids, amino acids, choline, ketone bodies, nucleotides, and lipids. Based on receiver operating characteristic (ROC) curve analysis and a biomarker panel with an area under the curve (AUC) of 0.969, six serum metabolites (α-glucose, choline, glutamine, glutamate, valine, and dihydrothymine) were selected as potential diagnostic biomarkers for early stage ESCC. Additionally, four potential PO biomarkers (α-glucose, pyruvate, glutamate, and valine) with an AUC of 0.985 were selected to distinguish ESCC and PO. Many metabolites trended towards normalisation in PO patients, with only choline remaining high with an AUC of 0.858, suggesting that it may be a valuable potential biomarker for neoplasm progression, recurrence, chemoradiotherapy, and prognosis. 1H-NMR spectroscopy may be a useful tumour detection approach in the early diagnosis of ESCC. These results also indicate that it is useful to differentiate pre- and post-operative ESCC, evaluate surgery therapeutic responses, and monitor postoperative chemoradiotherapy.

【 授权许可】

CC BY-NC   

【 预 览 】
附件列表
Files Size Format View
RO201910255048402ZK.pdf 3109KB PDF download
  文献评价指标  
  下载次数:21次 浏览次数:12次