Protein secondary structure determination by NMR Application with recombinant human cyclophilin

FEBS Letters

Spitzfaden, Claus¹  Wider, Gerhard¹  Wüthrich, Kurt¹  Memmert, Klaus²  Widmer, Hans² 

[1] Institut für Molekularbiologie und Biophysik, ETH Hönggerberg, CH-8093 Zürich, Switzerland;Präklinische Forschung, Sandoz Pharma AG, CH-4002 Basel, Switzerland

关键词: Protein structure;    Secondary structure;    Nuclear magnetic resonance;    NMR structure;    Cyclophilin;   

DOI  :  10.1016/0014-5793(91)80808-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is a unique trait of the NMR method for protein structure determination that a description of the polypeptide secondary structure can be obtained at an early stage and quite independently of the complete structure calculation. In this paper the procedures used for secondary structure determination are reviewed and placed in perspective relative to the other steps in a complete three-dimensional structure determination. As an illustration the identification of the regular secondary structure elements in human cyclophilin is described.

【 授权许可】

Unknown