The photocycle of bacteriorhodopsin immobilized in poly(vinyl alcohol) film

FEBS Letters

Varo, G.¹  Drabent, R.²  Bryl, K.² 

[1] Institute of Biophysics, Biological Research Center, 6701 Szeged, Hungary;Department of Physics and Biophysics, University of Agriculture and Technology, 10-957 Olsztyn, Poland

关键词: Bacteriorhodopsin;    Photocycle;    Photoelectric signal;    Oriented system;    bR;    bacteriorhodopsin;    pm;    purple membrane;    PVA;    poly(vinyl alcohol);    PERS;    protein electric response signal;   

DOI  :  10.1016/0014-5793(91)80726-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The kinetics of photoelectric and optical signals were measured on samples containing oriented purple membranes immobilized in a poly(vinyl alcohol) film and on purple membranes introduced into a PVA—H₂O mixture. The bacteriorhodopsin photocycle in the PVA—H₂O mixture was complete. The only observed changes were the slowing down of the optical and electrical signals in relation to the M₄₁₂-O₆₄₀ and O₆₄₀-bR^a11-trans steps. In the PVA film the O₆₄₀ intermediate disappeared and a negative photoelectric signal appeared.

【 授权许可】

Unknown