Effect of pH on the photoreaction cycles of bacteriorhodopsin

FEBS Letters

Kobayashi, Takayoshi²  Ikegami, Akira¹  Iwai, Jin-ichi²  Ohtani, Hiroyuki²  Uchiki, Hisao² 

[1] Institute of Physical and Chemical Research, Wako, Saitama 351, Japan;Department of Physics, University of Tokyo, Hongo, Tokyo 113, Japan

关键词: Bacteriorhodopsin;    Photocycle;    Picosecond laser photolysis;    Nanosecond laser photolysis Proton pumping;    Absorption spectrum;   

DOI  :  10.1016/0014-5793(83)81078-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photoreaction cycles of acidified bacteriorhodopsin (bR^acid ₆₀₅) at room temperature was studied by nanosecond and picosecond spectroscopy. A precursor of bathobacteriorhodopsin was found to be converted to bathobacteriorhodopsin within 30 ps. Spectra and formation times of batho- and lumibacteriorhodopsin are identical to those of bacteriorhodopsin in neutral suspension. However, lumibacteriorhodopsin lifetime is > 700 μs and the formation of metabacteriorhodopsin was not observed in low pH suspension. Thus, the decay process of lumibacteriorhodopsin plays an important role in the proton pump action.

【 授权许可】

Unknown