An NAD‐specific glutamate dehydrogenase from cyanobacteria Identification and properties

FEBS Letters

Chávez, S.¹  Candau, P.¹ 

[1] Departamento de Bioquimica Vegetal y Biologia Molecular e Instituto de Bioquímica Vegetal y Fotosintesis, Universidad de Sevilla y CSIC, Apartado 1113, E-41080 Sevilla, Spain

关键词: NAD-glutamate dehydrogenase;    Ammonium assimilation;    Cyanobacterium;    (Synechocystis sp. PCC 6803);    GDH;    glutamate dehydrogenase;    GS;    glutamine synthetase;    GOGAT;    glutamate synthase;    MTT;    3-(4;    5-dimethylthiazol-2-yl)-2;    5-diphenyltetrazolium-bromide;    PMS;    phenazine methosulphate;    PMSF;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/0014-5793(91)80719-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The unicellular cyanobacterium Synechocystis sp. PCC 6803 presents a hexameric NAD-specific glutamate dehydrogenase with a molecular mass of 295 kDa. The enzyme differs from the NADP-glutamate dehydrogenase found in the same strain and is coded by a different gene. NAD-glutamate dehydrogenase shows a high coenzyme specificity, catalyzes preferentially glutamate formation and presents K_m values for ammonium, NADH and 2-oxoglutarate of 4.5 mM, 50 μM and 1.8 mM respectively. An animating role for the enzyme is discussed.

【 授权许可】

Unknown