期刊论文详细信息
FEBS Letters | |
An NAD‐specific glutamate dehydrogenase from cyanobacteria Identification and properties | |
Chávez, S.1  Candau, P.1  | |
[1] Departamento de Bioquimica Vegetal y Biologia Molecular e Instituto de Bioquímica Vegetal y Fotosintesis, Universidad de Sevilla y CSIC, Apartado 1113, E-41080 Sevilla, Spain | |
关键词: NAD-glutamate dehydrogenase; Ammonium assimilation; Cyanobacterium; (Synechocystis sp. PCC 6803); GDH; glutamate dehydrogenase; GS; glutamine synthetase; GOGAT; glutamate synthase; MTT; 3-(4; 5-dimethylthiazol-2-yl)-2; 5-diphenyltetrazolium-bromide; PMS; phenazine methosulphate; PMSF; phenylmethylsulfonyl fluoride; | |
DOI : 10.1016/0014-5793(91)80719-J | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The unicellular cyanobacterium Synechocystis sp. PCC 6803 presents a hexameric NAD-specific glutamate dehydrogenase with a molecular mass of 295 kDa. The enzyme differs from the NADP-glutamate dehydrogenase found in the same strain and is coded by a different gene. NAD-glutamate dehydrogenase shows a high coenzyme specificity, catalyzes preferentially glutamate formation and presents Km values for ammonium, NADH and 2-oxoglutarate of 4.5 mM, 50 μM and 1.8 mM respectively. An animating role for the enzyme is discussed.
【 授权许可】
Unknown
【 预 览 】
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RO201912020295022ZK.pdf | 440KB | download |