Interaction of HIV‐1 with susceptible lymphoblastoid cells 1H NMR studies

FEBS Letters

Luciani, A.M.¹  Verani, P.²  Viti, V.¹  Rossi, G.B.²  Rosi, A.¹  Sulli, N.²  Federico, M.²  Guidoni, L.¹  Maggiorella, M.T.² 

[1] Laboratorio di Fisica e INFN Sez. Sanita, Istituto Superiore di Sanita, viale Regina Elena 299, 00161 Roma, Italy;Laboratorio di Virologia, Istituto Superiore di Sanita, viale Regina Elena 299, 00161 Roma, Italy

关键词: HIV-1;    Lymphoblastoid cell;    Membrane organization;    Lipid metabolism;    1H NMR spectroscopy;   

DOI  :  10.1016/0014-5793(91)80713-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Different strains of HIV susceptible lymphoblastoid cells have been infected by HIV-1 and examined by means of ¹H NMR spectroscopy at different times after infection, taking advantage of the presence of high resolution lipid signals from the plasma membrane of tumor cells. A transient decrease in intensity of fatty acid signals, originated by changes in membrane structure, has been observed early after viral infection. Marked alterations in membrane-dependent steps of phospholipid synthesis can also be inferred by the observed transient depression in peaks from choline-based metabolites. Spectral modifications deriving from charges in lipid metabolism are also produced both in infected cells a few days after infection and in permanently infected cells. ¹H NMR can, therefore, monitor structural and metabolic effects induced by HIV infection.

【 授权许可】

Unknown