Nucleosomes occurring in protein‐free hybridoma cell culture Evidence for programmed cell death

FEBS Letters

Dolníková, Jana¹  Franěk, František¹ 

[1] Institute of Molecular Genetics, Czechoslovak Academy of Science, Videňska 1083, CS-14220 Praha 4, Czechoslovakia

关键词: Nucleosome: Chromatin;    B hybridoma;    Cell culture;    Cell death;    Apoptosis;    NaCl/Pi;    phosphate-buffered saline;    pH 7.4;   

DOI  :  10.1016/0014-5793(91)80705-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In addition to monoclonal immunoglobulin. two kinds of nucleoproteins. NP1 and NP2, were isolated from the supernatants of hybridoma cultures set up in a protein-free medium. As shown by SDS-electrophoresis the two nucleoproteins shared a set or proteins (apparent M _r 11000 to 15000). and differed in the DNA moiety (∼ 150 bp in NP1. ∼ 350 bp in NP2). The amino acid composition of the protein moiety confirmed the nucleosomal origin of NP1 and NP2. The findings support the view that in hybridoma cultures the cells undergo death by apoptosis, i.e. a programmed process characterized by initial fragmentation of chromatin.

【 授权许可】

Unknown