Enrichment of bacteriochlorophyll g′ in membranes of Heliobacterium chlorum by ether extraction Unequivocal evidence for its existence in vivo

FEBS Letters

Kobayashi, Masami²  van de Meent, Erik Jan³  Amesz, Jan³  Ikegami, Isamu¹  Watanabe, Tadashi² 

[1] Department of Chemistry, Teikyo University, Kanagawa, Japan;Institute of Industrial Science, University of Tokyo, Tokyo, Japan;Department of Biophysics, University of Leiden, Leiden, The Netherlands

关键词: Heliobacterium reaction center;    Bacteriochlorophyll g′ Epimerization;    High-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(91)80778-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of H. chlorum membrane preparations with diethyl ether of high degrees of water saturation raised the bacteriochlorophyll (BChl) g′ mole fraction, as determined by HPLC analysis of their acetone extracts, toward a level of 40% of total BChl g or higher. Starting from pure BChl g, the BChl g′ mole fraction should never exceed 24.6% which is the equilibrium value in diethyl ether. The existence (and possible functioning) of BChl g′ in vivo is thus unequivocally demonstrated.

【 授权许可】

Unknown