Dystrophin constitutes 5% of membrane cytoskeleton in skeletal muscle

FEBS Letters

Ohlendieck, Kay¹  Cambell, Kevin P.¹ 

[1] Howard Hughes Medical Institute and Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, USA

关键词: Dystrophin;    Sarcolemma;    Membrane cytoskeleton;    Skeletal muscle;   

DOI  :  10.1016/0014-5793(91)80595-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dystrophin, which is absent in skeletal muscle of Duchenne muscular dystrophy patients, has not been considered to play a major structural role in the cell membrane of skeletal muscle because of its low abundance (∼ 0.002% of total muscle protein). Here, were have determined the relative abundance of dystrophin in a membrane cytoskeleton preparation and found that dystrophin constitutes approximately 5% of the total membrane cytoskeleton fraction of skeletal muscle sarcolemma. In addition, dystrophin can be removed from surcolemma by alkaline treatment. Thus, our results have demonstrated that dystrophin is a major component of the subsurcolemmal cytoskeleton in skeletal muscle and suggest that dystrophin could play a major structural role in the cell membrane of skeletal muscle.

【 授权许可】

Unknown