Molecular cloning and sequence analysis of cDNA encoding δ4‐3‐ketosteroid 5β‐reductase of rat liver

FEBS Letters

Okuda, Kyuichiro²  Noshiro, Mitsuhide²  Onishi, Yoshiaki¹  Shimosato, Tsunehiro¹ 

[1] Department of Oral and Maxillofacial Surgery II, Hiroshima University School of Denistry, Hiroshima 734, Japan;Department of Biochemistry, Hiroshima University School of Denistry, Hiroshima 734, Japan

关键词: δ4-3-Ketosteroid 5β-reductase;    cDNA cloning;   

DOI  :  10.1016/0014-5793(91)80591-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA clone encoding δ⁴-3-ketosteroid 5β-reductase was isolated from rat liver cDNA libraries using antibodies specific for the enzyme and oligonucleotides as probes. The cDNA contained 981-base pair open reading frame encoding 327 amino acid residues (M, 37 376) and an unusually long 3′-untranslated region rich in AT sequence in the total length of 3189 base pairs. The predicted amino acid sequence contains th sequences similar to the putative NADPH- and steroid-binding regions.

【 授权许可】

Unknown