The kinase insert domain of colony stimulating factor‐1 receptor is dispensable for CSF‐1 induced phosphatidylcholine hydrolysis

FEBS Letters

Pierce, Jacalyne¹  Sylvia, Victor L.²  Choudhury, Goutam Ghosh²  Sakaguchi, Alan Y.²  Wang, Ling-Mei² 

[1] Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA;Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX 78284-7762, USA

关键词: Colony stimulating factor-1 receptor;    Tyrosine kinase;    Phosphatidylcholine hydrolysis;    NIH 3T3 cell;    CSF1;    colony stimulating factor-1;    CSFIR;    colony stimulating factor-1 receptor;    DAG;    diacylglycerol;    EGFR;    epidermal growth factor receptor;    IP3;    inositol 1;    4;    5-trisphosphate;    KI;    kinase insert;    PC;    phosphatidylcholine;    PDGFR;    platelet-derived growth factor receptor;    PI;    phosphatidylinositol;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    PLC;    phospholipase C;    PMSF;    phenylmethylsulfonylfluoride;    Protein A;    Staphylococcus aureus membrane Protein A;    PtdIns 3-kinase;    phosphatidylinositol 3-kinase;    SDS;    sodium dodecyl sulfate;    TLC;    thin layer chromatography;    U;    units;   

DOI  :  10.1016/0014-5793(91)80511-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mouse NIH 3T3 fibroblasts transfected with human colony stimulating factor-1 receptor produced diacylglycerol in response to CSFI and this correlated with elevated phosphatidylcholine hydrolyzing activity measured in an in vitro assay. Treatment of cells with the isoflavone derivative genistein attenuated PC hydrolysis in vitro suggesting a role for CSF1R tyrosine kinase activity. A CSF1R mutant lacking 67 amino acids of the kinase insert domain, which may affect the association of receptor with certain substrates, stimulated PC hydrolysis in response to CSF1. Coupling to PC hydrolysis is likely a general property of CSFIR and the kinase insert domain is dispensable for this activity.

【 授权许可】

Unknown