Ca2+ Ionophore and Phorbol Ester Stimulate Diacylglycerol Formation and Phosphatidylcholine Hydrolysis in Rat Parotid Acinar Cells

The Japanese Journal of Pharmacology

Kazuhiro Suda¹  Takao Komabayashi¹  Atsushi Yakata¹  Masamichi Noguchi¹  Minoru Tsuboi¹  Tetsuya Izawa² 

[1] Department of Physiology and Pharmacology, Tokyo College of Pharmacy;Laboratory for Exercise and Applied Physiology, Tokyo College of Pharmacy

关键词: Parotid acinar cells;    A23187;    Phorbol 12;    13-dibutyrate;    sn-1;    2-Diacylglycerol accumulation;    Phosphatidylcholine hydrolysis;   

DOI  :  10.1254/jjp.59.97

学科分类：药理学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(33)Cited-By(1)We investigated the effects of A23187 and phorbol 12, 13-dibutyrate (PDBu) on sn-1, 2-diacylglycerol (DAG) accumulation and phosphatidylcholine (PC) hydrolysis in rat parotid acinar cells. Both A23187 and PDBu, in concentration ranges of 0.001-0.1 μM, stimulated DAG accumulation and PC hydrolysis in a time- and concentration-dependent manner. Treatment with A23187 and PDBu stimulated the release of [3H]choline and [3H]phosphocholine into the medium, indicating [3H]PC hydrolysis is due to the activation of phospholipases C and D; however, [3H]phosphatidylethanolamine hydrolysis was not indicated. These releases were unaffected by the addition of glucose 6-phosphate, a phosphatase inhibitor. Staurosporine, a protein kinase C inhibitor, significantly inhibited the DAG accumulation and the PC hydrolysis stimulated by these agents. Combinations of A23187 and PDBu potentiated the stimulatory effect which each of these agents alone had on DAG accumulation and PC hydrolysis. This mode of action was additive but not synergistic. These results suggest that DAG accumulation induced by A23187 and PDBu is related to the PC hydrolysis mediated via the activation of phospholipases C and D, and that it is not related to phosphatidylethanolamine hydrolysis.

【 授权许可】

Unknown