Regulation of acetylcholine receptor gene expression in rats treated with α‐bungarotoxin

FEBS Letters

Fuchs, Sara¹  Asher, Orna¹  Provenzano, Carlo¹ 

[1] Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: α-Bungarotoxin;    Acetylcholine receptor transcript;    Experimental autoimmune myasthenia gravis;    Denervation;    Myogenin;    MyoD1;   

DOI  :  10.1016/0014-5793(91)80487-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Regulation of acetylcholine receptor (AChR) gene expression was analyzed in α-bungarotoxin (α-BTX) treated rats. A reduction in available ¹²⁵l-α-BTX binding sites was accompanied by an increase in the various AChR transcripts. The increase in the AChR α-, β- c- and δ-subunit mRNAs was similar to that observed in rats with experimental autoimmune myasthenia gravis (EAMG). Unlike in EAMG, the γ-subunit transcripts reappeared following α-BTX treatment. The quantitative differences in the levels of AChR transcripts between α-BTX treatment and EAMG on one hand and denervation on the other hand, support the notion that the regulation of AChR gene expression is controlled by muscle activity and by neuronal factors as well. We also demonstrate in this report that myogenin transcripts increase following α-BTX treatment as well as following denervation, whereas MyoD1 transcripts remain stable.

【 授权许可】

Unknown