Increased levels of acetylcholine receptor α‐subunit mRNA in experimental autoimmune myasthenia gravis

FEBS Letters

Fuchs, Sara¹  Asher, Orna¹  Neumann, Drorit¹ 

[1] Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: Acetylcholine receptor;    Experimental autoimmune myasthenia gravis;    mRNA;   

DOI  :  10.1016/0014-5793(88)80442-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To gain insight into the regulatory mechanisms underlying the blockade and loss of acetylcholine receptor (AChR) in myasthenia, we have followed AChR α-subunit mRNA levels in leg muscles of myasthenic and normal rabbits and rats. Northern blots of RNA preparations from normal and myasthenic animals were hybridized with a mouse AChR α-subunit cDNA probe. Our experiments indicate a specific increase (4–7-fold) in the levels of α-subunit mRNA in animals with experimental autoimmune myasthenia gravis (EAMG), in comparison with control animals. Actin mRNA levels were essentially unchanged. Our results thus suggest that EAMG is accompanied by an increased level of AChR gene transcription.

【 授权许可】

Unknown