The ‘positive‐inside rule’ applies to thylakoid membrane proteins

FEBS Letters

Gavel, Ylva²  Steppuhn, Johannes¹  von Heijne, Gunnar¹  Herrmann, Reinhold¹ 

[1] Botanisches Institut der Ludwig-Maximilians-Universität, Menzinger Strasse 67, D-8000 München 19, Germany;Research Group for Theoretical Biophysics, Department of Theoretical Physics, Royal Institute of Technology, S-10044 Stockholm, Sweden

关键词: Thylakoid;    Membrane protein;    Topology;    Positive inside rule;   

DOI  :  10.1016/0014-5793(91)80440-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In integral membrane proteins, regions that span the lipid bilayer alternate with regions that are exposed on either side of the membrane. For proteins from the plasma membrane of both prokaryotic and eukaryotic cells it has been shown that the exposed parts follow a ‘positve-inside rule’: on average, segments that are translocated across the membrane have a 2–4-fold lower frequency of positively charged residues than non-translocated segments. We now present an analysis of proteins from the thylakoid membrane of chloroplasts. It is shown that these proteins have the same charge asymmetry as has been reported for proteins from other membrane systems, with their more highly charged regions facing the stromal compartment.

【 授权许可】

Unknown