FEBS Letters | |
D‐[35S(U)]Inositol 1,4,5‐trisphosphorothioate, a novel radioligand for the inositol 1,4,5‐trisphosphate receptor Complex binding to rat cerebellar membranes | |
Challiss, R.A.John2  Potter, Barry V.L.1  Nahorski, Stefan R.2  Smith, Susan M.2  | |
[1] School of Pharmacy and Pharmacology, University of Bath, Claverton Down, Bath, BA2 7AY, UK;Department of Pharmacology and Therapeutic, University of Leicester, Leicester, LE1 9HN, UK | |
关键词: Inositol 1; 4; 5-trisphosphate; Inositol 1; 4; 5-trisphosphorothioate; Inositol 1; 4; 5-trisphosphate receptor; | |
DOI : 10.1016/0014-5793(91)80368-D | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
D-[35S(U)]mya-inositol 1,4,5-trisphosphorothioate ([35S]InsPS3), a synthetic, metabolically stable analogue of inositol 1,4,5-trisphosphate (InsP3) binds with high affinity (Ka 58.6±9.1 nM) to rat cerebellar revealing a high density of specific binding sites (D max 21.5±2.1 pmol/mg of protein). Comparison with [3H]InsP3 binding reveals a higher density of sites labelled by [35S]InsPS3 and complex competition curves for displacement of specific [35S]insPS3 by InsP3. The results suggests that [35S]InsPS3 labels two sites in rat cerebellar membranes with equal affinity: the InsP3 receptor and a site that displays low affinity for InsP3.
【 授权许可】
Unknown
【 预 览 】
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