Biphasic expression of slow myosin light chains and slow tropomyosin isoforms during the development of the human quadriceps muscle

FEBS Letters

Laurent-Winter, C.²  Soussi-Yanicostas, N.¹  Butler-Browne, G.S.¹ 

[1] INSERM U262, Clinique Universitaire Bandelocque, 123 boulevard Port-Royal, 75014 Paris, France;Laboratoire d'électrophorèse bidimensionnelle, Institut Pasteur, 25 rue du Dr. Roux. 75724 Paris cedex 15, France

关键词: Myosin light chain;    Tropomyosin;    Development;    Human;    Muscle;    Two-dimensional electrophoresis;    2D-PAGE;    two-dimensional polyacrylamide gel electrophoresis;    MLC;    myosin light chain;    MHC;    myosin heavy chain;    MLC1S;    MLC2S;    slow myosin light chains;    MLC1F;    MLC2F;    MLC3F;    fast myosin light chains;    MLClemb;    embryonic myosin light chain;    αk TM;    slow tropomyosin isoform;    αr TM;    fast tropomyosin isoform;    β TM;    β tropomyosin isoform;   

DOI  :  10.1016/0014-5793(91)80315-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a two-dimensional electrophoresis technique coupled with sensitive silver staining, we have investigated the chronology of appearance of the myosin light chain and tropomyosin isoforms during early stages of human quadriceps development. Our results show that slow myosin light chains and the slow tropomyosin isoform are not detected at 6 weeks of gestation. These isoforms transiently appear between 12.5 weeks and 15 weeks of gestation and then disappear. The slow myosin light chains are re-expressed at 31 weeks of gestation and the slow tropomyosin isoform later at 36 weeks of gestation, and normally remained expressed into the adulthood. Our study thus reveals a biphasic expression of the slow myosin light chains and the slow tropomyosin isoform in developing human quadriceps muscle.

【 授权许可】

Unknown