The expression of interleukin‐6 by a rat macrophage‐derived cell line

FEBS Letters

Braciak, Todd A.³  Fey, George H.¹  Gauldie, Jack¹  Northemann, Wolfgang² 

[1] Department of Immunology, Research Institutes of Seripps Clinic, La Jolla, CA 92037, USA;Department of Molecular Biology, ELIAS Entwicklungslabor, D-7800 Freiburg, Germany;Department of Pathology, McMaster University, Hamilton, Ontario L8N3Z5, Canada

关键词: Interleukin-6;    Macrophage cell line;    Expression;    Regulation;    mRNA level;    Rat;    IL1;    interleukin-1;    IL6;    interleukin-6;    LPS;    lipopolysaccharide;    PECs;    peritoneal exuate cells;    TNF-α;    tumor necrosis factor α;   

DOI  :  10.1016/0014-5793(91)80311-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A stable rat macrophage-derived cell line (RMSV1) was established by transformation of primary peritoneal exudate cells with the SV40 virus. The RMSV1 cell line was used as a model to study the regulation of the interleukin-6 (IL6) gene expression in rate macrophages with respect to lipopolysaccharides (LPS), interleukin-1 (IL1) and glucocorticoids. The IL6 mRNA level in RMSV1 cell lines was induced 20-fold within 4 h by LPS, whereas IL1 had no effect. The glucocorticoids were able to inhibit completely the induction of the IL6 mRNA synthesis by LPS, indicating the negative regulation of the IL6 gene expression by glucocorticoids.

【 授权许可】

Unknown