Structure of adsorbed fibrinogen obtained by scanning force microscopy

FEBS Letters

Lundström, Ingemar¹  Welin, Stefan¹  Erlandsson, Ragnar¹  Elwing, Hans¹  Wigren, Roger¹ 

[1] Department of Physics and Measurement Technology, Linköping University, S-581 83 Linköping, Sweden

关键词: Molecular structure;    Fibrinogen;    Adsorption;    Scanning force microscopy;    Atomic force microscopy;    Polymerization;   

DOI  :  10.1016/0014-5793(91)80298-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It is shown that scanning force microscopy (SFM), operated in the attractive mode, can be used to obtain high resolution pictures of adsorbed fibrinogen molecules on solid surfaces, without the need for staining or special microscope grids. SFM also reveals the three-dimensional structure of the adsorbed molecules. Two forms of adsorbed fibrinogen are demonstrated on hydrophobic silicone dioxide surfaces; a trinodular about 60 nm long and a globular with about a 40 nm diameter. Polymeric networks formed after storage of the surface with adsorbed fibrinogen in PBS for 11 days are also shown. The SFM-results for the trinodular structure suggest the existence of loops or peptide chains extending outside the basic structure of the fibrinogen molecule.

【 授权许可】

Unknown