Expression of insulin‐like growth factor‐IA and factor‐IB mRNA in human liver, hepatoma cells, macrophage‐like cells and fibroblast

FEBS Letters

Nagaoka, Isao¹  Iwabuchi, Kazuhisa¹  Someya, Akimasa¹  Yamashita, Tatsuhisa¹ 

[1] Department of Biochemistry, Juntendo University, School of Medicine, Hongo, Bunkyo-ku, Tokyo 113, Japan

关键词: Insulin-like growth factor-I;    Alternative splicing;    mRNA;    Polymerase chain reaction;    RNase protection assay;    IGF-I;    insulin-like growth factor-I;    RT-PCR;    reverse transcription-polymerase chain reaction;    bp;    base pair;   

DOI  :  10.1016/0014-5793(91)80208-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The human insulin-growth factor-I (IGF-1) gene codes for two transcripts, IGF-IA and IGF-IB mRNAs, formed by alternative splicing. In this study, the expression of these IGF-I mRNA transcripts was examined using human liver, hepatoma cells, macrophage-like cells and fibroblasts. The reverse transcription-polymerase chain reaction revealed that these cells contained both IGF-IA mRNA (representing exons I, II, III and V) and IGF-IB mRNA (representing exons I, II, III and IV). Interestingly, an RNase protection assay using ³²P-labeled IGF-IA and IGF-IB exon-specific cRNA probes demonstrated that IGF-IA mRNA was 10-fold more abundant than IGF-IB mRNA in these cells. However, there was no difference in the stabilities of IGF-IA and IGF-IB mRNA. These observations indicate that IGF-IA mRNA is more expressed than IGF-IB mRNA in these cells independent of their stabilities.

【 授权许可】

Unknown