Isolation and functional expression of pituitary peptidylglycine α‐amidating enzyme mRNA

FEBS Letters

Yonekura, Hideto¹  Kato, Ichiro¹  Yamamoto, Hiroshi¹  Okamoto, Hiroshi¹ 

[1]Department of Biochemistry, Tohoku University School of Medicine. Sendai 980, Miyagi, Japan

关键词: Peptide α-amidating enzyme;    Functional expression;    Secretory form;    Transmembrane domain;    Alternative splicing;    mRNA;    kbp;    kilobase pairs;    bp;    base pairs;    Hepes;    4(2-hydroxyethyl)-1-piperazineethane sulfonic acid;   

DOI  :  10.1016/0014-5793(90)81184-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We demonstrate that, in rat pituitary, peptidylglycine α-amidating enzyme was encoded by at least 5 distinct mRNAs. Southern blot and ribonuclease protection analyses revealed that the mRNAs arose through alternative splicing. A variant lacking the transmembrane domain-coding sequence was a major mRNA species for the enzyme in the pituitary. When the cDNAs were expressed in COS-7 cells, the variant was the most efficient in producing a secretory form (37 kDA) of the enzyme.

【 授权许可】

Unknown