Demonstration of a transcription element in vitro between the capping site and translation initiation site of the mouse myelin basic protein gene

FEBS Letters

Mikoshiba, Katsuhiko¹  Tamura, Taka-aki¹ 

[1] Division of Behavior and Neurobiology, National Institute for Basic Biology, Myodaiji-cho, Okazaki-444, Japan

关键词: Transcription in vitro;    Transcriptional control;    Myelin basic protein;    MBP;    myelin basic protein;    MLP;    adenovirus major late promoter;   

DOI  :  10.1016/0014-5793(91)80207-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A transcription element was identified, by in vitro analyses, just downstream from the capping site of the mouse myelin basic protein (MBP) gene. Deletion of this element caused a dramatic drop of transcription efficiency in mouse brain, rat liver and HeLa cell nuclear extracts, regardless of the form of DNA being closed circular or linear form. DNase I footprint analysis demonstrated the presence of a ubiquitous trans-acting factor for this region. This element functioned even when it is located in the normal direction downstream from the adenovirus major late promoter. Mutation analysis suggested that an essential part of the downstream element was located between +25 and +45.

【 授权许可】

Unknown