Role of a GC‐rich motif in transcription regulation of the adenovirus type 2 IVa2 promoter which lacks typical TATA‐box element

FEBS Letters

Mikoshiba, Katsuhiko¹  Tamura, Taka-aki¹ 

[1] Division of Behavior and Neurobiology, National Institute for Basic Biology, Myodaiji-cho, Okazaki-444, Japan

关键词: Adenovirus type 2 IVa2 promoter;    Transcription in vitro;    Transcriptional regulation;    Spl;    DNase I footprint;    IVa2;    adenovirus type 2 IVa2 gene;    MLP;    adenovirus type 2 major late promoter;    bp;    base pairs;    PPE;    proximal promoter element;    DHFR;    dihydrofolate reductase;   

DOI  :  10.1016/0014-5793(91)80450-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Promoter of the adenovirus type 2 IVa2 (IVa2) gene does not have a TATA-box element, hence it is considered to be a model of cellular TATA-less promoters. In vitro transcription of the IVa2 promoter in HeLa cell extracts identified a proximal cis-element around −40 relative to the transcription start site. DNase I footprint analysis revealed the presence of a DNA-binding factor which interacts with sequences ACCCCTCCCACTTAG at −50. Competition footprint analysis and sequence comparisons showed that the IVa2 proximal promoter element is equivalent to the GC-box, the Spl-binding site.

【 授权许可】

Unknown