The repression of the reverse‐oriented transcription from the adenovirus terminus by NFI in competition with TFIID

FEBS Letters

Hanaoka, Fumio¹  Matsumoto, Ken³  Ui, Michio²  Nagata, Kyosuke³ 

[1] Radiation Biology Laboratory, RIKEN Institute, Wako, Saitama 351-01, Japan;Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Tokyo 113, Japan;Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan

关键词: Transcription repression;    Nuclear factor I;    TFIID;    Adenovirus;   

DOI  :  10.1016/0014-5793(91)80202-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nuclear factor 1 (NFI) represses the transcription which is promoted by the cloned adenovirus (Ad) type 5 DNA replication origin and is reverse-oriented with respect to the direction of the replication. The mechanism of this repression by NFI was investigated. In the cell-free transcription system, the repression was observed only when NFI was present during the formation of the transcription initiation complex. From the results of DNase I protection experiments, it was indicated that NFI bound to its binding site in the Ad replication origin prevents TFIID from proper binding to the adjacent AT-rich region and consequently represses the transcription.

【 授权许可】

Unknown