Effect of alanine insertion (P′5) on the reactive centre of α1‐antitrypsin

FEBS Letters

Carrell, Robin W.¹  Avron, Ann¹  Reeve, Francesca H.¹  Lickorish, Josephine M.¹ 

[1] Department of Haematology, University of Cambridge, Hills Road, Cambridge, CB2 2QL, UK

关键词: α1-Antitrypsin;    Serpin;   

DOI  :  10.1016/0014-5793(91)80199-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A recombinant mutant of α₁-antitrypsin with an inserted alanine in position P′₁ (362=363) was compared with wild-type recombinant and plasma α₁-antitrypsins. Initial studies showed that contrary to other reports the wild recombinant inhibitor had the same, or even greater, association constants with trypsin and clastasex as the plasma inhibitor. The recombinant mutant as predicted had decreased inhibitory activity but no significant alteration in denaturation temperature and it retained the typical serpin S-R change.

【 授权许可】

Unknown