Tyrosine protein phosphorylation is required for protein kinase C‐mediated proliferation in T cells

FEBS Letters

Zubiaga, Ana M.²  Muñoz, Eduardo³  Huber, Brigitte T.¹ 

[1] Department of Pathology, Tufts University Medical School, Boston, MA, USA;Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA, USA;Departamento de Bioquimica y Biologia Molecular, Facultad de Medicina de Cordoba, Cordoba Spain

关键词: Protien kinase C;    C-mye;    Tyrosine kinase;    Cell proliferation;    T cell;   

DOI  :  10.1016/0014-5793(91)80178-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the role of tyrosine kinase in PMA-stimulated T cells. Protein kinase C (PKC)-mediated D10A cell proliferation is inhibited by the specific inhibitor of tyrosine kinase, tyrphostin. This inhibitor selectively blocks the mRNA expression of the proto-oncogene e-mye in response to the phorbol ester. On the other hand, the same doses of this inhibitor do not affect the mRNA expression of the proto-oncogene c-for in PMA-stimulated D10A cells. Phorbol esters induce in this T cell line the tyrosine phosphorylation of a unique protein of 42 kDa and the enzyme PKC is required for this activity.

【 授权许可】

Unknown