Gibberellic acid stimulates acid invertase secretion in pea ovary protoplasts

FEBS Letters

Beltrán, José Pio¹  Estruch, Juan José¹ 

[1] Instituto de Agroquimica y Technologia de Alimentos (IATA), Consejo Superior de Investigaciones Cientificas (CSIC), Jaime Roig 11, 46010 Valencia, Spain

关键词: Gibberellie acid;    Acid invertase;    Secretion;    Protoplast;    Pisum sativum L;    BSA;    bovine serum albumin;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    CCR;    cyanide-insensitive NADH-cytochrome c reductase;    DCCD;    N;    N′-dicyclohexyl carbodiimine;    FCCP;    carbonyl cyanide 4-trifluoromethoxyphenylhydrazone;    GA3;    gibberellic acid;    GAPDh;    glyceraldehyde-3-phosphate dehydrogenase;    IDP-ase;    inosine diphosphatase;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0014-5793(91)80173-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protoplasts purified from mesocarp of nonpollinated pea (Pisum sativum L.) ovaries released acid invertase to the incubation. The association of the acid invertase with microsomal fractions, and the sensitivity to energy-metabolism inhibitors and to lunicamycin, indicated the secretory nature of the release process. In the presence of GA₃ (10 μM), the protoplasts increased their invertase secretion at about 60 min. this effect being counteracted by lunicamycin but not cycloheximide. Subcellular fractionation of GA₃-treated protoplasts showed that higher invertase secretion was the result of a promotion of invertase transfer from endoplasmic reticulum (ER) to Golgi apparatus.

【 授权许可】

Unknown