Identification of a receptor protein for neuropeptide Y in rabbit kidney G‐protein association and inhibition of adenylate cyclase

FEBS Letters

Lang, Rudolf E.¹  Gimpl, Gerald¹  Wahl, Jochen¹ 

[1] Department of Pharmacology and the German Institute for High Blood Pressure Research, University of Heidelberg, Im Neuenheimer Feld 366, D6900 Heidelberg, Germany

关键词: Neuropeptide Y;    Peptide receptor;    Crosslinking;    cAMP;   

DOI  :  10.1016/0014-5793(91)80153-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We previously identified the receptor for neuropeptide Y (NPY) on rabbit kidney membranes as a 100 kDa protein [(1990) J. Biol. Chem. 265, 18142–18143]. It is demonstrated in this study that the stable guanine nucleotide analogue, guanosine 5′-O-(3-thiotriphosphate)(GTP_γS), is capable of decreasing the labeling of the 100 kDa protein in a concentration-dependent manner, with an IC₂₀ value of 50 μM. This suggests that the 100 kDa protein represents a G-protein coupled receptor type. In isolated proximal tubulex from rabbit kidney NPY decreases the parathyroid hormone stimulated cAMP production in a dose-dependent fashion. This indicates that the NPY receptor in rabbit kidney is negatively coupled to adenylate cyclase by a G₁-like protein.

【 授权许可】

Unknown